evaluation of an immunoblotting test based on whole tachyzoite lysate for diagnosis of humoral response of domestic cats infected with toxoplasma gondii, rh strain
نویسندگان
چکیده
this study was aimed to detect anti-toxoplasma gondii antibodies in cats infected with rh strain of t. gondii by using an immunoblotting method. for this, cats were experimentally infected using tachyzoites harvested from vero cell cultures. tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to pvdf membranes. an immunoblotting was performed using these membranes to detect igg antibodies. protein bands were detected in regions related to the presence of t. gondii surface antigens, dense granular or rhoptry proteins. no positive bands were detected in serum samples of kittens that received only vero cell lysates (controls). positive or negative results were in concordance to the results of ifat. the results of this study revealed that whole tachyzoite antigen based immunoblotting is an appropriate diagnostic test for serological detection of anti-t. gondii antibodies in recently infected kittens with this apicomplexan parasite.
منابع مشابه
Evaluation of an immunoblotting test based on whole tachyzoite lysate for diagnosis of humoral response of domestic cats infected with Toxoplasma gondii, Rh strain
This study was aimed to detect anti-Toxoplasma gondii antibodies in cats infected with Rh strain of T. gondii by using an immunoblotting method. For this, cats were experimentally infected using tachyzoites harvested from Vero cell cultures. Tachyzoites were then lysed and transferred to polyacrylamide gels followed by blotting to PVDF membranes. An immunoblotting was performed using these mem...
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Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). Cats are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibo...
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در سالهای اخیر،اختلالات کیفیت توان مهمترین موضوع می باشد که محققان زیادی را برای پیدا کردن راه حلی برای حل آن علاقه مند ساخته است.امروزه کیفیت توان در سیستم قدرت برای مراکز صنعتی،تجاری وکاربردهای بیمارستانی مسئله مهمی می باشد.مشکل ولتاژمثل شرایط افت ولتاژواضافه جریان ناشی از اتصال کوتاه مدار یا وقوع خطا در سیستم بیشتر مورد توجه می باشد. برای مطالعه افت ولتاژ واضافه جریان،محققان زیادی کار کرده ...
15 صفحه اولCyst Formation from Virulent RH Strain of Toxoplasma gondii Tachyzoite: In Vitro Cultivation
BACKGROUND This study was performed to induce conversion of RH strain tachyzoites of Toxoplasma gondii to bradyzoites by pH changing of the culture medium. METHODS HeLa cell monolayers were infected at a 1:1 tachyzoite to cell ratio. Four hours after infection, the culture medium was removed and replaced with culture medium and 5% FCS, adjusted to pH 8 with NaOH. The culture was maintained at...
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متن کاملEvaluation of Humoral Immune Response of Cats to the Experimental Infection with the different Clonal Types of Toxoplasma gondii by Measurement of IgG Antibodies
Toxoplasma gondii is one of the most prevalent parasitic infections in world. Rh, NED and Me49 are of the most prevalent clonal types of the parasite isolated till now. Differences in pathogenicity and virulence of different types have been investigated in different studies. No controlled study was performed to compare the ability of different types to initiate humoral immune response. We inves...
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عنوان ژورنال:
iranian journal of veterinary researchناشر: shiraz university
ISSN 1728-1997
دوره 14
شماره 1 2013
کلمات کلیدی
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